Streptavidin bind to biotin with high affinity. Streptavidin conjugates with fluorophores are widely used as a secondary labeling method to detect biotinylated molecules in common research applications such as:
• flow cytometry
• immunofluorescence microscopy
• in situ hybridization
• western blot, and other applications.
Amid Biosciences provides several popular streptavidin conjugates with fluorophores: Streptavidin-488 FluoroDye, Streptavidin-568 FluoroDye and Streptavidin-647 FluoroDye conjugates.