Amid Biosciences | Protein Engineering Company
SARS-CoV-2 Spike S1 Protein, Recombinant, AviTag | Amid Biosciences
The coronavirus Spike protein (S protein) is a large oligomeric transmembrane protein that mediates coronavirus entry into host cells. It contains S1 and S2 subunits. The coronavirus first binds to a receptor on the host cell surface through Spike S1 subunit, and then fuses viral and host membranes through Spike S2 subunit. S1 subunit contains N-terminal domain (NTD), receptor binding domain (RBD), and receptor binding motif (RBM).
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with the 8XHis tag and AviTag at the N-terminus.
Expression host: Escherichia coli (E. coli).
Expressed Region of S1: Val16 – Arg685
Predicted molecular weight: 78.1 kDa
Tags: His, AviTag
Purity: > 90% as analyzed by SDS-PAGE
Storage buffer: 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT and 50% Glycerol.
Storage: The product can be stored at -20°C or below. Avoid repeated freezing and thawing cycles. The shelf life of the product is unspecified.
This product is intended for laboratory research use only.
Image of 3D print of a spike protein on the surface of SARS-CoV-2 (Credit: the NIH 3D Print Exchange at 3dprint.nih.gov; NIH)