{"title":"Recombinant Human Sphingolipid Activator Proteins (Saposins)","description":"\u003cp\u003e\u003cstrong\u003eSphingolipid Activator Proteins (SAPs)\u003c\/strong\u003e, collectively known as \u003cstrong\u003eSaposins\u003c\/strong\u003e, are small, non-enzymatic, heat-stable glycoproteins localized primarily within the lysosomal compartment. Derived from a single precursor protein, prosaposin, the four distinct mature domains—\u003cstrong\u003eSaposin A, B, C, and D\u003c\/strong\u003e—serve as essential biological cofactors. They structurally reorganize lipid membranes and physically present sphingolipids to specific hydrolytic enzymes for degradation. Dysregulation or deficiency in individual saposins is heavily implicated in lysosomal storage diseases, metabolic disorders, and neurodegenerative pathways, making high-purity recombinant variants invaluable tools for therapeutic development.\u003c\/p\u003e\n\u003ch3\u003eKey Research \u0026amp; Assay Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLipid Metabolism \u0026amp; Enzymology Studies:\u003c\/strong\u003e Ideal for reconstitution assays investigating lysosomal hydrolase activity and lipid-protein biophysics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Delivery Systems:\u003c\/strong\u003e Used in the engineering of biocompatible saposin-lipoprotein nanoparticles (e.g., SapC-DOPS) for selective, membrane-targeted therapeutic delivery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOncology \u0026amp; Translational Research:\u003c\/strong\u003e Essential reagents for analyzing tumor microenvironment interactions, apoptosis induction, and biomarker validation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNeuroscience \u0026amp; Biomarker Discovery:\u003c\/strong\u003e Vital tools for profiling sphingolipid accumulation profiles in neurodegenerative disease models.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProduct Availability\u003c\/h3\u003e\n\u003cp\u003eAmid Biosciences offers a comprehensive portfolio of high-purity, low-endotoxin \u003cstrong\u003erecombinant human Saposins (A, B, C, and D)\u003c\/strong\u003e expressed in optimized bacterial systems to ensure robust structural integrity and reproducible assay performance.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003csmall\u003e\u003cem\u003eAll products in this collection are intended for laboratory research use only (RUO).\u003c\/em\u003e\u003c\/small\u003e\u003c\/strong\u003e\u003c\/p\u003e","products":[{"product_id":"recombinant-human-saposin-a-protein","title":"Saposin A, Human, Recombinant","description":"\u003cp\u003e\u003cstrong\u003eSaposin A\u003c\/strong\u003e is a small, highly stable, cysteine-rich alpha-helical glycoprotein localized within the lysosomal compartment. Derived from the prosaposin precursor protein, it serves as an essential non-enzymatic cofactor required for the lysosomal degradation of galactosylceramide by β-galactosylceramidase. While native saposins are glycosylated, non-glycosylated recombinant saposins retain their full biological activation properties in functional \u003cem\u003ein vitro\u003c\/em\u003e assays, making them exceptional tools for membrane biophysics.\u003c\/p\u003e\n\u003ch3\u003eKey Applications: Lipid Nanoparticle Scaffolding\u003c\/h3\u003e\n\u003cp\u003eBeyond its native role in sphingolipid catabolism, recombinant Saposin A is widely utilized as a scaffolding protein in versatile lipid nanoparticle systems (saposin-lipoprotein discs). These engineered scaffolds allow researchers to reconstitute challenging membrane proteins and transporters into a native-like lipid bilayer. This technology is highly valued across structural biology (including cryo-EM determination), targeted therapeutic delivery of protein-based pharmacological agents, and vaccine adjuvant formulation.\u003c\/p\u003e\n\u003ch3\u003eProduct Features \u0026amp; Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression Host:\u003c\/strong\u003e Produced in an optimized \u003cem\u003eEscherichia coli\u003c\/em\u003e (\u003cem\u003eE. coli\u003c\/em\u003e) bacterial expression system.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag Status:\u003c\/strong\u003e Expressed as an N-terminal His-tag fusion and purified using proprietary chromatography steps, followed by site-specific proteolytic cleavage to yield a completely \u003cstrong\u003etag-free\u003c\/strong\u003e final protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Purity:\u003c\/strong\u003e Verified at \u0026gt;90% purity by Coomassie blue staining on SDS-PAGE.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation \u0026amp; Concentration:\u003c\/strong\u003e Supplied at 1.0 – 2.0 mg\/mL in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 50% Glycerol for premium stability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003csmall\u003e\u003cem\u003eCatalog Number: SAPA-301 | \u003cstrong\u003eThis product is intended for laboratory research use only (RUO)\u003c\/strong\u003e. Shipping on ice packs required. \u003c\/em\u003e\u003c\/small\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":12475835154513,"sku":"SAPA-301-1","price":600.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59537114955857,"sku":"SAPA-301-2","price":2800.0,"currency_code":"USD","in_stock":true},{"title":"10.0 mg","offer_id":59537117184081,"sku":"SAPA-301-10","price":7500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Saposin_A_Human_Recombinant.png?v=1779503974"},{"product_id":"saposin-c-human-recombinant-his-tag","title":"Saposin C, Human, Recombinant, His-tag","description":"\u003cp\u003e\u003cstrong\u003eSaposin C\u003c\/strong\u003e (SAP-C) is a small, heat-stable lysosomal glycoprotein derived from the prosaposin precursor protein via site-specific proteolytic cleavage. Among the four prosaposin-derived domains, Saposin C shares the highest structural and sequence homology with Saposin A. It acts as an indispensable biological cofactor required for the lysosomal activation of β-glucocerebrosidase (GBA), the enzyme deficient in Gaucher disease. Additionally, Saposin C plays a critical role in cellular immunology by facilitating the presentation of lipid antigens through CD1b molecules to human T cells. While native lysosomal saposins are heavily glycosylated, non-glycosylated recombinant variants expressed in bacterial systems maintain full biological activation properties in functional \u003cem\u003ein vitro\u003c\/em\u003e biochemical assays.\u003c\/p\u003e\n\u003ch3\u003eProduct Features \u0026amp; Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression System:\u003c\/strong\u003e Engineered and expressed in an optimized \u003cem\u003eEscherichia coli\u003c\/em\u003e (\u003cem\u003eE. coli\u003c\/em\u003e) bacterial host.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFusion Tag:\u003c\/strong\u003e Features a C-terminal Polyhistidine (6xHis) tag to allow robust detection, tracking, and oriented immobilization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Purity:\u003c\/strong\u003e Verified at \u0026gt;90% purity by Coomassie blue staining on SDS-PAGE.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation \u0026amp; Concentration:\u003c\/strong\u003e Supplied at a concentration of 1.0 mg\/mL (determined by A280) in 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 50% Glycerol for premium structural stability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eValidated Assay Applications\u003c\/h3\u003e\n\u003cp\u003eThis recombinant human Saposin C construct is specifically optimized for lysosomal enzyme activity assays, \u003cem\u003ein vitro\u003c\/em\u003e glucocerebrosidase reconstitution kinetics, lipid-binding biophysical studies, CD1b lipid antigen presentation assays, Surface Plasmon Resonance (SPR), and Bio-Layer Interferometry (BLI).\u003c\/p\u003e\n\u003cp\u003e\u003csmall\u003e\u003cem\u003eCatalog Numbers: SAPC-301-1 (0.1 mg) | SAPC-301-2 (1.0 mg). \u003cstrong\u003eThis product is intended for laboratory research use only (RUO).\u003c\/strong\u003e It is not intended for diagnostic or therapeutic use in humans or animals. \u003c\/em\u003e\u003c\/small\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":12475963768913,"sku":"SAPC-301-1","price":600.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59537121280081,"sku":"SAPC-301-2","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Saposin_C_Human_Recombinant_His-tag.png?v=1779505009"},{"product_id":"recombinant-human-saposin-b-protein","title":"Saposin B, Human, Recombinant","description":"\u003cp\u003e\u003cstrong\u003eSaposin B\u003c\/strong\u003e (SAP-B) is a small, non-enzymatic glycosphingolipid activator protein essential for the lysosomal degradation of cerebroside sulfates (sulfatides). It functions as a biological detergent, physically extracting target lipid molecules from biological membranes to form a soluble, transportable protein-lipid complex. This presentation renders the substrate accessible to the hydrolytic enzyme arylsulfatase A (ARSA). Deficiencies or genetic mutations in the prosaposin gene leading to a loss of functional Saposin B result in Saposin B deficiency, a rare lysosomal storage disease phenotypically resembling metachromatic leukodystrophy (MLD).\u003c\/p\u003e\n\u003ch3\u003eKey Research \u0026amp; Assay Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme Replacement \u0026amp; Activity Assays:\u003c\/strong\u003e Ideal as a functional cofactor for reconstituting \u003cem\u003ein vitro\u003c\/em\u003e arylsulfatase A degradation kinetics and lipid editing mechanics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunology \u0026amp; NKT Cell Activation:\u003c\/strong\u003e Used in downstream investigations profiling phospholipid transfer dynamics and lipid antigen presentation to Natural Killer T (NKT) cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug Discovery Support:\u003c\/strong\u003e High-value target component for screening therapeutics, small molecules, or chaperone designs aiming to mitigate metachromatic leukodystrophy pathways.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProduct Features \u0026amp; Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression System:\u003c\/strong\u003e Engineered and produced in an optimized \u003cem\u003eEscherichia coli\u003c\/em\u003e (\u003cem\u003eE. coli\u003c\/em\u003e) bacterial expression platform.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag Status:\u003c\/strong\u003e Expressed as an N-terminal His-tag fusion and purified using proprietary chromatography steps, followed by site-specific proteolytic cleavage to yield a completely \u003cstrong\u003etag-free\u003c\/strong\u003e final protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSequence Profile:\u003c\/strong\u003e Comprises the native 79-amino acid mature Saposin B sequence (GDVCQDCIQM...CDE).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Purity:\u003c\/strong\u003e Formulated to \u0026gt;90% purity as verified by Coomassie blue staining on SDS-PAGE.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffer Formulation:\u003c\/strong\u003e Supplied at a concentration of 0.5 – 2.0 mg\/mL in 25 mM Phosphate Buffer, pH 7.2, 75 mM NaCl, and 50% Glycerol for premium structural stability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003csmall\u003e\u003cem\u003eCatalog Number: SAPB-301 | \u003cstrong\u003eThis product is intended for laboratory research use only (RUO)\u003c\/strong\u003e. Long-term storage is recommended at -20°C. \u003c\/em\u003e\u003c\/small\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":32396239110225,"sku":"SAPB-301-1","price":600.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59537119248465,"sku":null,"price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Saposin_B_Human_Recombinant.png?v=1779504561"},{"product_id":"recombinant-human-saposin-c-protein","title":"Saposin C, Human, Recombinant","description":"\u003cp\u003e\u003cstrong\u003eSaposin C\u003c\/strong\u003e (SAP-C) is a small, heat-stable lysosomal glycoprotein derived from the prosaposin precursor protein via site-specific proteolytic cleavage. Among the four prosaposin-derived domains, Saposin C shares high structural homology with Saposin A and acts as an indispensable biological cofactor required for the lysosomal activation of β-glucocerebrosidase (GBA)—the enzyme deficient in Gaucher disease. Additionally, Saposin C plays a critical role in cellular immunology by facilitating the presentation of lipid antigens through CD1b molecules to human T cells. While native lysosomal saposins are heavily glycosylated, non-glycosylated recombinant variants expressed in bacterial systems maintain full biological activation properties in functional \u003cem\u003ein vitro\u003c\/em\u003e biochemical assays.\u003c\/p\u003e\n\u003ch3\u003eKey Applications \u0026amp; SapC-DOPS Nanocarriers\u003c\/h3\u003e\n\u003cp\u003eBeyond its role in sphingolipid catabolism, recombinant Saposin C is widely utilized in translational oncology research. It can be assembled with dioleoylphosphatidylserine to form \u003cstrong\u003eSapC-DOPS\u003c\/strong\u003e, a highly selective, membrane-targeted nanocarrier platform used to target and induce apoptosis in various cancer models, including glioblastoma multiforme. This makes high-purity, tag-free Saposin C an essential reagent for drug delivery innovation, lipid metabolism studies, and vaccine adjuvant formulation.\u003c\/p\u003e\n\u003ch3\u003eProduct Features \u0026amp; Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression System:\u003c\/strong\u003e Engineered and expressed in an optimized \u003cem\u003eEscherichia coli\u003c\/em\u003e (\u003cem\u003eE. coli\u003c\/em\u003e) bacterial host.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag Status:\u003c\/strong\u003e Expressed as an N-terminal His-tag fusion and purified using proprietary chromatography steps, followed by site-specific proteolytic cleavage to yield a completely \u003cstrong\u003etag-free\u003c\/strong\u003e final protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Purity:\u003c\/strong\u003e Verified at \u0026gt;90% purity by Coomassie blue staining on SDS-PAGE.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSequence Profile:\u003c\/strong\u003e Native mature Saposin C sequence (SDVYCEVCEF...SG).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation \u0026amp; Storage:\u003c\/strong\u003e Supplied at a concentration of 1.0 mg\/mL (determined by A280) in 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 50% Glycerol. Store securely at -20°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003csmall\u003e\u003cem\u003eCatalog Number: SAPC-302-1 | \u003cstrong\u003eThis product is intended for laboratory research use only (RUO).\u003c\/strong\u003e Product requires shipping on ice packs.\u003c\/em\u003e\u003c\/small\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":40280463605841,"sku":"SAPC-302-1","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Saposin_C_Human_Recombinant.png?v=1779506672"},{"product_id":"recombinant-human-saposin-d-protein","title":"Saposin D, Human, Recombinant","description":"\u003cp\u003e\u003cstrong\u003eSaposin D\u003c\/strong\u003e (SAP-D) is a small, heat-stable lysosomal glycoprotein derived from the prosaposin precursor protein via site-specific proteolytic cleavage. It functions as an indispensable non-enzymatic cofactor required for the lysosomal breakdown of ceramides into free fatty acids and sphingosine by acid ceramidase (ASAH1). Deficiencies in functional Saposin D or its cognate enzyme lead to severe ceramide accumulation, causing lysosomal storage disorders such as Farber lipogranulomatosis. While native saposins feature complex glycosylation patterns, non-glycosylated recombinant saposins expressed in bacterial systems retain full biological activation properties in functional \u003cem\u003ein vitro\u003c\/em\u003e biochemical and lipid reconstitution assays.\u003c\/p\u003e\n\u003ch3\u003eKey Research \u0026amp; Assay Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCeramide Catabolism \u0026amp; Enzymology:\u003c\/strong\u003e Functions as a vital cofactor for reconstituting \u003cem\u003ein vitro\u003c\/em\u003e acid ceramidase enzyme kinetics and lipid-cleaving mechanics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLipidomics \u0026amp; Membrane Biophysics:\u003c\/strong\u003e Used in downstream structural biochemistry profiling to study protein-lipid bilayer interactions and membrane permeabilization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTherapeutic \u0026amp; Chaperone Screening:\u003c\/strong\u003e High-value target component for screening small molecule chaperones, enzyme replacement therapies (ERT), or drug designs targeting Farber disease pathways.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProduct Features \u0026amp; Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression System:\u003c\/strong\u003e Engineered and produced in an optimized \u003cem\u003eEscherichia coli\u003c\/em\u003e (\u003cem\u003eE. coli\u003c\/em\u003e) bacterial expression platform.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag Status:\u003c\/strong\u003e Expressed as an N-terminal His-tag fusion and purified using proprietary chromatography steps, followed by site-specific proteolytic cleavage to yield a completely \u003cstrong\u003etag-free\u003c\/strong\u003e final protein.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Purity:\u003c\/strong\u003e Formulated to \u0026gt;90% purity as verified by Coomassie blue staining on SDS-PAGE.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage Buffer:\u003c\/strong\u003e Supplied at a concentration of 0.5 – 2.0 mg\/mL in 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 50% Glycerol for premium structural stability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003csmall\u003e\u003cem\u003eCatalog Number: SAPD-301-1 | \u003cstrong\u003eThis product is intended for laboratory research use only (RUO). \u003c\/strong\u003eProduct requires shipping on ice packs.\u003c\/em\u003e\u003c\/small\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":40280464851025,"sku":"SAPD-301-1","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Saposin_D_Human_Recombinant.png?v=1779506289"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/collections\/Saposin_A_Human_Recombinant.png?v=1779503382","url":"https:\/\/amidbiosciences.com\/collections\/saposins-recombinant-human-saposins-sap-proteins.oembed","provider":"Amid Biosciences","version":"1.0","type":"link"}