{"title":"Recombinant RAS \u0026 KRAS Mutant Proteins | Amid Biosciences","description":"\u003cp\u003eRAS proteins are crucial in cancer research due to their role in cellular signal transduction. As a family of small GTPases, RAS proteins—including HRAS, KRAS, and NRAS—are among the most common oncogenes in human cancers. KRAS mutations, notably \u003ca href=\"https:\/\/amidbiosciences.com\/products\/kras-g12c-protein-human-recombinant-biotinylated?_pos=5\u0026amp;_sid=306552204\u0026amp;_ss=r\" title=\"Recombinant Human KRAS G12C Protein, Biotinylated | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eG12C\u003c\/a\u003e, \u003ca href=\"https:\/\/amidbiosciences.com\/products\/kras-g12d-protein-human-recombinant-biotinylated?_pos=1\u0026amp;_psq=G12D\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"Recombinant Human KRAS G12D Protein, Biotinylated | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eG12D\u003c\/a\u003e, \u003ca rel=\"noopener\" title=\"Recombinant Human KRAS G12D Protein, Biotinylated | Amid Biosciences\" href=\"https:\/\/amidbiosciences.com\/products\/kras-g12v-protein-human-recombinant-biotinylated?_pos=2\u0026amp;_psq=G12V\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003eG12V\u003c\/a\u003e, \u003ca href=\"https:\/\/amidbiosciences.com\/products\/kras-g13d-protein-human-recombinant-biotinylated?_pos=2\u0026amp;_psq=G13D\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"Recombinant Human KRAS G13D Protein, Biotinylated | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eG13D\u003c\/a\u003e, and \u003ca rel=\"noopener\" title=\"Recombinant Human KRAS Q61H Protein, Biotinylated | Amid Biosciences\" href=\"https:\/\/amidbiosciences.com\/products\/kras-q61h-protein-human-recombinant-biotinylated?_pos=2\u0026amp;_psq=Q61H\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003eQ61H\u003c\/a\u003e, are highly prevalent in tumors, making them vital targets for oncology drug discovery, including the development of small-molecule inhibitors and molecular glues.\u003c\/p\u003e\n\u003cp\u003eAmid Biosciences supports this critical research area by offering an extensive range of high-purity recombinant biotinylated and non-biotinylated RAS proteins, including \u003ca rel=\"noopener\" title=\"Recombinant Human KRAS Wild Type Protein | Amid Biosciences\" href=\"https:\/\/amidbiosciences.com\/products\/kras-protein-human-recombinant-biotinylated?_pos=1\u0026amp;_psq=KRAS+W\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003ewild-type \u003c\/a\u003eand mutant KRAS, \u003ca href=\"https:\/\/amidbiosciences.com\/products\/hras-protein-human-biotinylated?_pos=1\u0026amp;_psq=HRAS\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"Recombinant Human HRAS Wild Type Protein, Biotinylated | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eHRAS\u003c\/a\u003e, and \u003ca rel=\"noopener\" title=\"Recombinant Human NRAS Wild Type Protein, Biotinylated | Amid Biosciences\" href=\"https:\/\/amidbiosciences.com\/products\/nras-protein-human-biotinylated?_pos=1\u0026amp;_psq=NRAS\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003eNRAS\u003c\/a\u003e GTPases. Our proteins are engineered as essential tools for binding assays, structural biology, and high-throughput screening (HTS). Additionally, we provide custom services for loading RAS proteins with specific nucleotides (GTP\/GDP) or their analogs to fit your precise experimental needs.\u003c\/p\u003e\n\u003cp\u003eTo complement your RAS signal transduction pathway research, we also provide key regulatory biomolecules, effector domains, and upstream\/downstream targets, including:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/amidbiosciences.com\/products\/raf1-rbd-50-140-protein-human-recombinant-biotinylated?_pos=2\u0026amp;_psq=Raf1-RBD\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"Raf1 RBD Protein, Human, Recombinant, Biotinylated | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eRaf1-RBD\u003c\/a\u003e, \u003ca rel=\"noopener\" title=\"PI3Kα-RBD Protein, Human, Biotinylated | Amid Biosciences\" href=\"https:\/\/amidbiosciences.com\/products\/pi3k-rbd-protein-human-biotinylated?_pos=1\u0026amp;_psq=PI3K%CE%B1-RBD\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003ePI3Kα-RBD\u003c\/a\u003e, and \u003ca href=\"https:\/\/amidbiosciences.com\/products\/ralgds-rbd-human-mbp-tag?_pos=1\u0026amp;_psq=RALGDS-RBD\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"RalGDS-RBD, human, MBP-tag | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eRALGDS-RBD\u003c\/a\u003e \u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"SOS1 Protein, Human, Recombinant | GTP Exchange | GEF Activity |Amid Biosciences\" href=\"https:\/\/amidbiosciences.com\/products\/sos1-protein-human-recombinant?_pos=1\u0026amp;_psq=SOS1\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003eSOS1 \u003c\/a\u003e(GEF domain) and \u003ca href=\"https:\/\/amidbiosciences.com\/products\/neurofibromin-nf1-human-recombinant?_pos=1\u0026amp;_psq=Neurofibromin\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"Neurofibromin (NF1), Human, Recombinant | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eNeurofibromin \u003c\/a\u003e(NF1-GRD)\u003c\/li\u003e\n\u003cli\u003e\n\u003ca rel=\"noopener\" title=\"SHP2 Protein, Human, Recombinant, Biotinylated | Amid Biosciences\" href=\"https:\/\/amidbiosciences.com\/products\/shp2-protein-human-recombinant-biotinylated?_pos=1\u0026amp;_psq=SHP2%2C\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003eSHP2,\u003c\/a\u003e \u003ca href=\"https:\/\/amidbiosciences.com\/products\/rala-protein-human-recombinant-biotinylated?_pos=1\u0026amp;_psq=RALA\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"RalA Protein, Human, Recombinant, Biotinylated | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eRALA\u003c\/a\u003e, \u003ca rel=\"noopener\" title=\"RalB Protein, Human, Recombinant, Biotinylated | Amid Biosciences\" href=\"https:\/\/amidbiosciences.com\/products\/ralb-protein-human-recombinant-biotinylated?_pos=1\u0026amp;_psq=RALB\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003eRALB\u003c\/a\u003e, \u003ca href=\"https:\/\/amidbiosciences.com\/products\/rheb-protein-human-recombinant-biotinylated?_pos=1\u0026amp;_psq=Rheb\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"Rheb Protein, Human, Recombinant, Biotinylated | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eRheb\u003c\/a\u003e, \u003ca rel=\"noopener\" title=\"MEK1 Protein, Human, Recombinant, Biotinylated | Amid Biosciences\" href=\"https:\/\/amidbiosciences.com\/products\/mek1-protein-human-recombinant-biotinylated?_pos=1\u0026amp;_psq=MEK1\u0026amp;_ss=e\u0026amp;_v=1.0\" target=\"_blank\"\u003eMEK1\u003c\/a\u003e, and \u003ca href=\"https:\/\/amidbiosciences.com\/products\/mek2-protein-human-recombinant-biotinylated?_pos=1\u0026amp;_psq=MEK2\u0026amp;_ss=e\u0026amp;_v=1.0\" title=\"MEK2 Protein, Human, Recombinant, Biotinylated | Amid Biosciences\" rel=\"noopener\" target=\"_blank\"\u003eMEK2\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eValidated Application \u0026amp; Citation:\u003c\/strong\u003e\u003cbr\u003eOur biotinylated KRAS mutant proteins and SOS1 have been successfully utilized in high-throughput nucleotide exchange assays to characterize next-generation KRAS inhibitors and degraders.\u003c\/p\u003e\n\u003cp\u003eFor examples of these products in validated protocols, see:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eKidane M, Hoffman RM, Wolfe-Demarco JK, et al. Suite of Biochemical and Cell-Based Assays for the Characterization of Kirsten Rat Sarcoma (KRAS) Inhibitors and Degraders. \u003c\/span\u003e\u003ci\u003eACS Pharmacol Transl Sci\u003c\/i\u003e\u003cspan\u003e. 2024;7(12):3921-3934.  doi:1\u003ca rel=\"noopener\" title=\"Suite of Biochemical and Cell-Based Assays for the Characterization of Kirsten Rat Sarcoma (KRAS) Inhibitors and Degraders\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsptsci.4c00450\" target=\"_blank\"\u003e0.1021\/acsptsci.4c00450\u003c\/a\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cmeta charset=\"utf-8\"\u003e \u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eMorstein J, Bowcut V, Fernando M, et al. Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket. \u003c\/span\u003e\u003ci\u003eCell\u003c\/i\u003e\u003cspan\u003e. 2024;187(22):6379-6392.e17. doi:\u003ca href=\"https:\/\/www.cell.com\/cell\/fulltext\/S0092-8674(24)00908-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0092867424009085%3Fshowall%3Dtrue\" title=\"Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket\" target=\"_blank\"\u003e10.1016\/j.cell.2024.08.017\u003c\/a\u003e\u003c\/span\u003e\u003cbr\u003e\u003cb id=\"docs-internal-guid-f46b2f44-7fff-7194-401f-fd339c19608f\"\u003e\u003c\/b\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eKage M.; Hayashi R.; Matsuo A.; et al. Structure-activity relationships of middle-size cyclic peptides, KRAS inhibitors derived from an mRNA display. \u003c\/span\u003e\u003ci\u003eBioorg Med Chem\u003c\/i\u003e\u003cspan\u003e. \u003cstrong\u003e2024\u003c\/strong\u003e;110:117830. doi:\u003ca rel=\"noopener\" title=\"Structure-activity relationships of middle-size cyclic peptides, KRAS inhibitors derived from an mRNA display\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S096808962400244X?via%3Dihub\" target=\"_blank\"\u003e10.1016\/j.bmc.2024.117830\u003c\/a\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003csmall\u003e\u003cem\u003eFor Research Use Only (RUO). These products are intended solely for laboratory research and development and are not for diagnostic or therapeutic use in humans or animals.\u003c\/em\u003e\u003c\/small\u003e\u003c\/p\u003e","products":[{"product_id":"recombinant-human-kras-protein-avitag","title":"Wild Type KRAS Protein, Human, Recombinant, AviTag","description":"\u003cp\u003eHuman KRAS protein is a small guanosine triphosphatase (GTPase) that is the component of the mitogen activated protein kinase (MAPK) signaling pathway. High occurrences of KRAS mutations in some types of cancers make KRAS one of the most important targets in oncology for drug development.\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":31944706261073,"sku":"KRAS-301-1","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Wild_Type_KRAS_Protein_Human_Recombinant_AviTag.png?v=1736453657"},{"product_id":"kras-g12c-protein-human-recombinant-avitag","title":"KRAS G12C Protein, Human, Recombinant, AviTag","description":"\u003cp\u003eHuman KRAS protein is a small guanosine triphosphatase (GTPase) that is the component of the mitogen activated protein kinase (MAPK) signaling pathway. High occurrences of KRAS mutations in some types of cancers make KRAS one of the most important targets in oncology for drug development. \u003cspan\u003eT\u003c\/span\u003e\u003cspan\u003ehe four most frequent KRAS mutations are \u003c\/span\u003e\u003cspan\u003eG12D, G12V, G13D, and G12C.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":31944897560657,"sku":"KRAS12C-301","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12C_Protein_Human_Recombinant_AviTag.png?v=1736453897"},{"product_id":"kras-g12d-protein-human-recombinant-avitag","title":"KRAS G12D Protein, Human, Recombinant, AviTag","description":"\u003cp\u003eHuman KRAS protein is a small guanosine triphosphatase (GTPase) that is the component of the mitogen activated protein kinase (MAPK) signaling pathway. High occurrences of KRAS mutations in some types of cancers makeÊKRASÊone of the most important targets in oncology for drug development.\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":31944909848657,"sku":"KRAS12D-301","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12D_Protein_Human_Recombinant_AviTag.png?v=1736453577"},{"product_id":"kras-g12d-protein-human-recombinant-biotinylated","title":"KRAS G12D Protein, Human, Recombinant, Biotinylated","description":"\u003cp\u003eRecombinant \u003cstrong\u003eKRAS G12D \u003c\/strong\u003eprotein with the N-terminal AviTag\u003cmeta charset=\"utf-8\"\u003e\u003cb data-index-in-node=\"18\" data-path-to-node=\"6,0,0\"\u003e™\u003c\/b\u003e peptide and His-tag is produced in \u003cem\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca rel=\"noopener\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotinylation\" target=\"_blank\"\u003eBirA enzyme. \u003c\/a\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":32374587588689,"sku":"KRAS12D-B-301-01","price":800.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59460791205969,"sku":"KRAS12D-B-301-1","price":6000.0,"currency_code":"USD","in_stock":true},{"title":"0.05 mg","offer_id":59460791238737,"sku":"KRAS12D-B-301-005","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12D_Protein_Human_Recombinant_Biotinylated.png?v=1736453375"},{"product_id":"kras-g12c-protein-human-recombinant-biotinylated","title":"KRAS G12C Protein, Human, Recombinant, Biotinylated | Amid Bio","description":"\u003cp\u003eRecombinant \u003cstrong\u003eKRAS G12C \u003c\/strong\u003eprotein with N-terminal AviTag\u003cmeta charset=\"utf-8\"\u003e™ and His-tag is produced in \u003cem\u003eE. coli \u003c\/em\u003ecells and site-specifically biotinylated at the AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotinylation\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\" target=\"_blank\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e\u003cspan\u003e This product is for research use only. It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.05 mg","offer_id":39531486117969,"sku":"KRAS12C-B-301-005","price":500.0,"currency_code":"USD","in_stock":true},{"title":"0.1 mg","offer_id":59460791074897,"sku":"KRAS12C-B-301-01","price":800.0,"currency_code":"USD","in_stock":true},{"title":"1.00 mg","offer_id":59460791107665,"sku":"KRAS12C-B-301-1","price":6000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12C_Protein_Human_Recombinant_Biotinylated.png?v=1736455145"},{"product_id":"kras-g13d-protein-human-recombinant-avitag","title":"KRAS G13D Protein, Human, Recombinant, AviTag","description":"\u003cp\u003eHuman KRAS protein is a small guanosine triphosphatase (GTPase) that is the component of the mitogen activated protein kinase (MAPK) signaling pathway. High occurrences of KRAS mutations in some types of cancers makeÊKRASÊone of the most important targets in oncology for drug development. The four most frequent KRAS mutations areÊ\u003cspan\u003eG12D, G12V, G13D, and G12C.\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":32395727470673,"sku":"KRAS13D-301","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G13D_Protein_Human_Recombinant_AviTag.png?v=1736454887"},{"product_id":"kras-g12v-protein-human-recombinant-avitag","title":"KRAS G12V Protein, Human, Recombinant, AviTag","description":"\u003cp\u003eHuman KRAS protein is a small guanosine triphosphatase (GTPase) that is the component of the mitogen activated protein kinase (MAPK) signaling pathway. High occurrences of KRAS mutations in some types of cancers makeÊKRASÊone of the most important targets in oncology for drug development.\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":32395782651985,"sku":"KRAS12V-301","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12V_Protein_Human_Recombinant_AviTag.png?v=1736454199"},{"product_id":"kras-protein-human-recombinant-biotinylated","title":"Wild Type KRAS Protein, Human, Recombinant, Biotinylated","description":"\u003cp data-mce-fragment=\"1\"\u003eRecombinant \u003cstrong data-mce-fragment=\"1\"\u003eKRAS \u003c\/strong\u003eprotein with the N-terminal AviTagª peptide and His-tag is produced in \u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\" target=\"_blank\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.05 mg","offer_id":39531488903249,"sku":"KRAS-301-B-005","price":500.0,"currency_code":"USD","in_stock":true},{"title":"0.1 mg","offer_id":59460791009361,"sku":"KRAS-301-B-01","price":800.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59460791042129,"sku":"KRAS-301-B-1","price":6000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Wild_Type_KRAS_Protein_Human_Recombinant_Biotinylated.png?v=1736453028"},{"product_id":"kras-q61h-protein-human-recombinant-avitag","title":"KRAS Q61H Protein, Human, Recombinant, AviTag","description":"\u003cp style=\"background: white; margin: 0in 0in 11.25pt 0in;\" data-mce-fragment=\"1\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; color: #080707;\" data-mce-fragment=\"1\"\u003eRecombinant \u003cstrong style=\"box-sizing: border-box;\" data-mce-fragment=\"1\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\" data-mce-fragment=\"1\"\u003eKRAS Q61H \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003eprotein with the N-terminal AviTag peptide and His-tag is produced in \u003cem style=\"box-sizing: border-box;\" data-mce-fragment=\"1\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\" data-mce-fragment=\"1\"\u003eE. coli\u003c\/span\u003e\u003c\/em\u003e cells. Presence of AviTag peptide is useful for a site-specific biotinylation with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\" target=\"_blank\"\u003eBirA enzyme\u003c\/a\u003e. \u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":39336321024081,"sku":"KRAS61H-301","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_Q61H_Protein_Human_Recombinant_AviTag.png?v=1736454786"},{"product_id":"kras-q61h-protein-human-recombinant-biotinylated","title":"KRAS Q61H Protein, Human, Recombinant Biotinylated | Amid Biosciences","description":"\u003cp\u003eOur recombinant Q61H protein is produced with a dual-tag system (\u003cstrong\u003eHis-Tag and AviTag™\u003c\/strong\u003e) to ensure maximum flexibility in the lab. The site-specific biotinylation provides a uniform orientation on streptavidin surfaces, which is critical for maintaining the accessibility of the mutated switch II region during high-throughput screening (\u003cstrong\u003eHTS\u003c\/strong\u003e) using \u003cstrong\u003eAlphaLISA \u003c\/strong\u003eor \u003cstrong\u003eTR-FRET\u003c\/strong\u003eassays. It also allows to study high-affinity binding to RAF1, PI3K, and RAL GDS via \u003cstrong\u003eSPR (Biacore)\u003c\/strong\u003e or \u003cstrong\u003eBLI (Octet)\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eAvailable in 0.1 mg and \u003cstrong\u003e1.0 mg bulk packaging \u003c\/strong\u003efor scalable assay development.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCatalog # KRAS61H-B-301\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThis product is for laboratory research use only. \u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":39336330592337,"sku":"KRAS61H-B-301","price":800.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59460790878289,"sku":"KRAS61H-B-301-2","price":6000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_Q61H_Protein_Human_Recombinant_Biotinylated.png?v=1736454129"},{"product_id":"kras-g12v-protein-human-recombinant-biotinylated","title":"KRAS G12V Protein, Human, Recombinant, Biotinylated | Amid Biosciences","description":"\u003cp\u003eRecombinant \u003cstrong\u003eKRAS G12V \u003c\/strong\u003eprotein\u003cstrong\u003e \u003c\/strong\u003ewith the N-terminal AviTag™ peptide and His-tag is produced in \u003cem\u003eE. coli \u003c\/em\u003ecells and site-specifically biotinylated at AviTag with \u003ca rel=\"noopener\" title=\"BirA Biotin Protein Ligase, Recombinant | Specific Enzymatic Biotinylation\" href=\"https:\/\/amidbiosciences.com\/products\/recombinant-bira-biotin-ligase-in-vitro-biotinylation\" target=\"_blank\"\u003eBirA enzyme.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCatalog # KRAS12V-B-301\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAvailable in 0.1 mg and \u003cstrong\u003e1.0 mg bulk packaging\u003c\/strong\u003e for scalable assay development\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThis product is for laboratory research use only. \u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":39336335409233,"sku":"KRAS12V-B-301-01","price":800.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59460790812753,"sku":"KRAS12V-B-301-1","price":6000.0,"currency_code":"USD","in_stock":true},{"title":"0.05 mg","offer_id":59460790845521,"sku":"KRAS12V-B-301-005","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12V_Protein_Human_Recombinant_Biotinylated.png?v=1736453151"},{"product_id":"kras-g13d-protein-human-recombinant-biotinylated","title":"KRAS G13D Protein, Human, Recombinant, Biotinylated","description":"\u003cp\u003eRecombinant \u003cstrong\u003eKRAS G13D \u003c\/strong\u003eprotein\u003cstrong\u003e \u003c\/strong\u003ewith the N-terminal AviTag™ peptide and His-tag is produced in \u003cem\u003eE. coli \u003c\/em\u003ecells and a site-specifically biotinylated with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\" title=\"BirA Enzyme\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCatalog # KRAS13D-B-301\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThis product is for laboratory research use only.\u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":39336337080401,"sku":"KRAS13D-B-301-1","price":800.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59460790714449,"sku":"KRAS13D-B-301-2","price":6000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G13D_Protein_Human_Recombinant_Biotinylated.png?v=1736454044"},{"product_id":"raf1-rbd-protein-human-recombinant","title":"Raf1 RBD Protein, Human, Recombinant","description":"\u003cp data-mce-fragment=\"1\"\u003eHuman Ras Binding Domain (RBD) of \u003cstrong\u003eRaf-1 Proto-Oncogene\u003c\/strong\u003e, Serine\/Threonine Kinase (RAF1), (UniProt # P04049, amino acids 1-149), expressed with an N-terminal 6XHis and glutathione S-transferase (GST) dual tag in E. coli. The dual 6xHis-GST tag allows immobilization of \u003cstrong\u003eRaf1 RBD Protein\u003c\/strong\u003e and RBD-Ras complexes on either Ni-NTA or glutathione agarose resin columns. \u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":39336367489105,"sku":"RAF1-301-1","price":280.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59537104109649,"sku":"RAF1-301-2","price":2200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Raf1_RBD_Protein_Human_Recombinant.png?v=1736282184"},{"product_id":"kras-g12r-protein-human-recombinant-biotinylated","title":"KRAS G12R Protein, Human, Recombinant, Biotinylated","description":"\u003cp data-mce-fragment=\"1\"\u003eRecombinantÊ\u003cstrong data-mce-fragment=\"1\"\u003eKRAS G12RÊ\u003c\/strong\u003eprotein\u003cstrong data-mce-fragment=\"1\"\u003eÊ\u003c\/strong\u003ewith the N-terminal AviTagª peptide and His-tag is produced inÊ\u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotinylation\" target=\"_blank\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | High Activity\" rel=\"noopener\"\u003eBirA enzyme.\u003c\/a\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":39737816776785,"sku":"KRAS12R-B-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12R_Protein_Human_Recombinant_Biotinylated.png?v=1736453804"},{"product_id":"kras-g12r-protein-human-recombinant-avitag","title":"KRAS G12R Protein, Human, Recombinant, AviTag","description":"\u003cp data-mce-fragment=\"1\"\u003eRecombinantÊ\u003cstrong data-mce-fragment=\"1\"\u003eKRAS G12RÊ\u003c\/strong\u003eprotein\u003cstrong data-mce-fragment=\"1\"\u003eÊ\u003c\/strong\u003ewith the N-terminal AviTagª peptide and His-tag is produced inÊ\u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells. Presence of AviTagª peptide can be useful for a site-specific biotinylation with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\" target=\"_blank\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":39737822511185,"sku":"KRAS12R-301","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12R_Protein_Human_Recombinant_AviTag.png?v=1736454709"},{"product_id":"kras-y96d-protein-human-recombinant-avitag","title":"KRAS Y96D  Protein, Human, Recombinant, AviTag","description":"\u003cp data-mce-fragment=\"1\"\u003eRecombinantÊ\u003cstrong data-mce-fragment=\"1\"\u003eKRAS Y96DÊ\u003c\/strong\u003eprotein\u003cstrong data-mce-fragment=\"1\"\u003eÊ\u003c\/strong\u003ewith the N-terminal AviTagª peptide and His-tag is produced inÊ\u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells. Presence of AviTagª peptide can be useful for a site-specific biotinylation with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\" target=\"_blank\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":39737852362833,"sku":"KRAS96D-301","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_Y96D_Protein_Human_Recombinant_AviTag.png?v=1736454572"},{"product_id":"kras-y96d-protein-human-recombinant-biotinylated","title":"KRAS Y96D Protein, Human, Recombinant, Biotinylated","description":"\u003cp data-mce-fragment=\"1\"\u003eRecombinantÊ\u003cstrong data-mce-fragment=\"1\"\u003eKRAS Y96DÊ\u003c\/strong\u003eprotein\u003cstrong data-mce-fragment=\"1\"\u003eÊ\u003c\/strong\u003ewith the N-terminal AviTagª peptide and His-tag is produced inÊ\u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotinylation\" target=\"_blank\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\"\u003eBirA enzyme.\u003c\/a\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":39737859407953,"sku":"KRAS96D-B-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_Y96D_Protein_Human_Recombinant_Biotinylated.png?v=1736454509"},{"product_id":"sos1-protein-human-recombinant","title":"SOS1 Protein, Human, Recombinant | GTP Exchange | GEF Activity |Amid Biosciences","description":"\u003cp\u003e\u003cstrong\u003eSOS1\u003c\/strong\u003e, also known as son of sevenless homolog 1, is a Ras and Rac guanine nucleotide exchange factor (GEF). SOS1 binds and activates GDP-bound RAS family proteins at its catalytic binding site and in this way promotes exchange of GDP for GTP. Depletion of SOS1 or specific genetic inactivation of its GEF function has been shown to decrease the survival of tumor cells harboring a KRAS mutation (1).\u003c\/p\u003e\n\u003cp\u003eThe recombinant protein (amino acids 563-1049 of human SOS1) contains two domains, REM (Ras Exchanger Motif) and CDC25 (cell division cycle 25). CDC25 activates Ras and promotes nucleotide exchange on Ras proteins (2). The REM domain contains an activating allosteric site which binds Ras-GTP leading to additional stimulation of the CDC25 domain and potentiating the Ras-GTP exchange stimulated by SOS1.\u003c\/p\u003e\n\u003cp\u003eThe human SOS1 (Lys563-Thr1049) fragment with a polyhistidine tag at the C-terminus was expressed in E. coli.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eStudy inhibitors of SOS1 GTP exchange activity\u003c\/li\u003e\n\u003cli\u003eIdentification of SOS1 binding proteins\u003c\/li\u003e\n\u003cli\u003eStudy of SOS1 GEF activity with different GTPases\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eCatalog# SOS1-301\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cstrong data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 700; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);' data-complete=\"true\" data-sfc-cb=\"\" data-sfc-root=\"ep\" class=\"Yjhzub\"\u003eValidated Application:\u003c\/strong\u003e \u003c\/strong\u003eOur recombinant SOS1 protein was successfully utilized in high-throughput nucleotide exchange assays to characterize next-generation KRAS inhibitors and degraders.\u003cstrong\u003e \u003c\/strong\u003eRead the full protocol in: \u003cmeta charset=\"utf-8\"\u003e\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);' data-complete=\"true\" data-sfc-cb=\"\" data-sfc-root=\"ep\" data-sfc-cp=\"\" class=\"T286Pc\"\u003e\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);'\u003e\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);' data-complete=\"true\" data-ved=\"2ahUKEwjH-ajE7aKVAxXNyOYEHfjrHoMQ0fERegYIAAgTEAI\" data-sfc-cb=\"\" data-sfc-root=\"c\" data-sfc-cp=\"\" class=\"\"\u003eKidane, M.\u003c\/span\u003e\u003c\/span\u003e;\u003cspan\u003e \u003c\/span\u003e\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);'\u003e\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);' data-complete=\"true\" data-ved=\"2ahUKEwjH-ajE7aKVAxXNyOYEHfjrHoMQ0fERegYIAAgTEAM\" data-sfc-cb=\"\" data-sfc-root=\"c\" data-sfc-cp=\"\" class=\"\"\u003eHoffman, R. M.\u003c\/span\u003e\u003c\/span\u003e; Wolfe-Demarco, J. K.; et al. Suite of Biochemical and Cell-Based Assays for the Characterization of Kirsten Rat Sarcoma (KRAS) Inhibitors and Degraders.\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cem data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);' data-complete=\"true\" data-sfc-cb=\"\" data-sfc-root=\"ep\" class=\"eujQNb\"\u003e\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);' data-complete=\"true\" data-sfc-cb=\"\" data-sfc-root=\"ep\" data-sfc-cp=\"\" class=\"T286Pc\"\u003e\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);'\u003e\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);' data-complete=\"true\" data-ved=\"2ahUKEwjH-ajE7aKVAxXNyOYEHfjrHoMQ0fERegYIAAgTEAQ\" data-sfc-cb=\"\" data-sfc-root=\"c\" data-sfc-cp=\"\" class=\"\"\u003eACS Pharmacol. Transl. Sci.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 700; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);' data-complete=\"true\" data-sfc-cb=\"\" data-sfc-root=\"ep\" class=\"Yjhzub\"\u003e2024\u003c\/strong\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cem data-copy-service-computed-style='font-family: \"Google Sans\", Roboto, Arial, sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);' data-complete=\"true\" data-sfc-cb=\"\" data-sfc-root=\"ep\" class=\"eujQNb\"\u003e7\u003c\/em\u003e\u003cspan\u003e (12), 3921–3934. DOI: \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Suite of Biochemical and Cell-Based Assays for the Characterization of Kirsten Rat Sarcoma (KRAS) Inhibitors and Degraders\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsptsci.4c00450\" target=\"_blank\"\u003e10.1021\/acsptsci.4c00450.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThis product is for research use only. \u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":39751737081937,"sku":"SOS1-301-1","price":350.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59537138090065,"sku":"SOS1-301-2","price":1800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/SOS1_Protein_Human_Recombinant.png?v=1736281913"},{"product_id":"shp2-protein-human-recombinant-biotinylated","title":"SHP2 Protein, Human, Recombinant, Biotinylated | Amid Biosciences","description":"\u003cp\u003eSHP2, a Src homology 2 (SH2) domain containing non-transmembrane protein tyrosine phosphatase (PTP), is involved in a variety of signal transduction processes, such as the Ras-Raf-MAP kinase, Jak-Stat, and PI3 kinase pathways (1). Experimental data have also indicated SHP2 as a direct activator of Ras and a potential therapeutic target for cancers driven by oncogenic or hyperactive Ras variants (2, 3).\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eAvailable in 0.1 mg and \u003cstrong\u003e1.0 mg bulk packaging\u003c\/strong\u003e for scalable assay development.\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003e \u003c\/strong\u003eThis product is for research use only. It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40021299822673,"sku":"SHP2-301-0.1","price":800.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59537124130897,"sku":"SHP2-B-301-1","price":5000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/SHP2-Protein-Human-Recombinant-Biotinylated.png?v=1780523165"},{"product_id":"g12d-kras-1-166-biotinylated-at-the-c-terminus","title":"G12D KRAS (1-166), Biotinylated at the C-terminus","description":"\u003cp data-mce-fragment=\"1\"\u003eRecombinantÊ\u003cstrong data-mce-fragment=\"1\"\u003eKRAS G12DÊ\u003c\/strong\u003eprotein\u003cstrong data-mce-fragment=\"1\"\u003eÊ\u003c\/strong\u003ewith the \u003cstrong\u003eC-terminal AviTagª\u003c\/strong\u003e peptide and \u003cstrong\u003eHis-tag\u003c\/strong\u003e is produced inÊ\u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotinylation\" target=\"_blank\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\"\u003eBirA enzyme.\u003c\/a\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40168306507857,"sku":"KRAS12D-B-C-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/G12D_KRAS_1-166_Biotinylated_at_the_C-terminus.png?v=1736453437"},{"product_id":"g12c-kras1-166-biotinylated-at-the-c-terminus","title":"G12C KRAS(1-166), Human, Recombinant, Biotinylated at the C-terminus | Amid Biosciences","description":"\u003cp\u003e\u003cstrong\u003eRecombinant human KRAS G12C\u003c\/strong\u003e with cysteine (C) substituted for glycine-12 (G12). RecombinantÊ\u003cstrong data-mce-fragment=\"1\"\u003eKRAS G12CÊ\u003c\/strong\u003eprotein\u003cstrong data-mce-fragment=\"1\"\u003eÊ\u003c\/strong\u003ewith the C-terminal AviTagª peptide and His-tag is produced inÊ\u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\" title=\"BirA Biotin Ligase | In-vitro biotinylation | Site-specific | High Activity\" rel=\"noopener\" target=\"_blank\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40168381186129,"sku":"KRAS12C-B-C-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/G12C_KRAS_1-166_Biotinylated_at_the_C-terminus.png?v=1736453239"},{"product_id":"kras-1-166-protein-biotinylated-at-the-c-terminus","title":"Wild Type KRAS (1-166) Protein, Biotinylated at the C-terminus","description":"\u003cp data-mce-fragment=\"1\"\u003eRecombinant wild-type (WT) KRAS protein with the C-terminal AviTagª peptide and His-tag is produced in \u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Efficiency\" rel=\"noopener\" target=\"_blank\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40168385806417,"sku":"KRAS-B-C-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Wild_Type_KRAS_1-166_Protein_Biotinylated_at_the_C-terminus.png?v=1736453729"},{"product_id":"raf1-rbd-50-140-protein-human-recombinant-biotinylated","title":"Raf1 RBD Protein, Human, Recombinant, Biotinylated","description":"\u003cp data-mce-fragment=\"1\"\u003eRas Binding Domain (RBD) of Human Raf-1 Proto-Oncogene, Serine\/Threonine Kinase (RAF1), (UniProt # P04049, amino acids 50-140) with the N-terminal AviTagª peptide and His-tag is produced inÊ\u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca rel=\"noopener\" title=\"BirA Biotin Ligase | In-vitro biotinylation | Site-specific | High Efficiency\" href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\" target=\"_blank\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40221648781393,"sku":"RAF1-B-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Raf1_RBD_Protein_Human_Recombinant_Biotinylated.png?v=1736282036"},{"product_id":"g12v-kras1-166-biotinylated-at-the-c-terminus","title":"G12V KRAS(1-166), Biotinylated at the C-terminus","description":"\u003cp data-mce-fragment=\"1\"\u003eRecombinantÊ\u003cstrong data-mce-fragment=\"1\"\u003eKRAS G12VÊ\u003c\/strong\u003eprotein\u003cstrong data-mce-fragment=\"1\"\u003eÊ\u003c\/strong\u003ewith the C-terminal AviTagª peptide and His-tag is produced inÊ\u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotinylation\" target=\"_blank\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\"\u003eBirA enzyme.\u003c\/a\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40287281971281,"sku":"KRAS12V-B-C-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/G12V_KRAS_1-166_Biotinylated_at_the_C-terminus.png?v=1736453966"},{"product_id":"rheb-protein-human-recombinant-biotinylated","title":"Rheb Protein, Human, Recombinant, Biotinylated","description":"\u003cp\u003eRheb (\u003cspan\u003e\u003cu\u003eR\u003c\/u\u003e\u003c\/span\u003easÊ\u003cspan\u003e\u003cu\u003eh\u003c\/u\u003e\u003c\/span\u003eomologÊ\u003cspan\u003e\u003cu\u003ee\u003c\/u\u003e\u003c\/span\u003enriched inÊ\u003cspan\u003e\u003cu\u003eb\u003c\/u\u003e\u003c\/span\u003erain) is a small GTPase that functions as an important mediator between the tumor suppressor proteins TSC1. Rheb shares about 30Ð40% sequence identity with members of the Ras\/Rap subfamily of small GTPases. Human Rheb consists of 184 amino acid residues. The 169 N-terminal residues form the GTPase domain; the 15 C-terminal residues are hypervariable with a flexible structure and comprise a conserved carboxyl CAA\u003cem\u003eX\u003c\/em\u003eÊmotif that plays important roles in the farnesylation of Rheb and its association with membrane (1).\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40329244999761,"sku":"RHEB-B-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Rheb_Protein_Human_Recombinant_Biotinylated.png?v=1736282329"},{"product_id":"rala-protein-human-recombinant-biotinylated","title":"RalA Protein, Human, Recombinant, Biotinylated","description":"\u003cp\u003eRas-related protein Ral-A (RALA) is a member of the Ras GTPaseÊfamily and shares a high structural similarity with RAS proteins (1). In human cells, the two protein isoforms, RALA and RALB, have been shown to play roles in the proliferation, survival, and metastasis of a variety of human cancers. RALA and RALB share 81% sequence homology with one another and more than 50% sequence homology with RAS. Given their importance in cancer biology, RAL proteins have emerged as desirable clinical targets (2).\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40355811033169,"sku":"RALA-B-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/RalA_Protein_Human_Recombinant_Biotinylated.png?v=1736282252"},{"product_id":"ralb-protein-human-recombinant-biotinylated","title":"RalB Protein, Human, Recombinant, Biotinylated","description":"\u003cp\u003eRas-related protein Ral-B (RALB) is a member of the Ras GTPaseÊfamily and shares a high structural similarity with RAS proteins (1). In human cells, the two protein isoforms, RALA and RALB, have been shown to play roles in the proliferation, survival, and metastasis of a variety of human cancers. RALA and RALB share 81% sequence homology with one another and more than 50% sequence homology with RAS. Given their importance in cancer biology, RAL proteins have emerged as desirable clinical targets (2).\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40355811229777,"sku":"RALB-B-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/RalB_Protein_Human_Recombinant_Biotinylated.png?v=1736282491"},{"product_id":"mek1-protein-human-recombinant-biotinylated","title":"MEK1 Protein, Human, Recombinant, Biotinylated | Amid Biosciences","description":"\u003cp\u003eMEK1 is a dual specificity protein kinase, which is known as a mitogen-activated protein (MAP) kinase kinase 1 (MAPKK 1). MAP kinases are recognized as extracellular signal-regulated kinases (ERKs) that act as an integration position for multiple biochemical signals. MEK1 is located upstream of MAP kinases and catalyzes phosphorylation of threonine and tyrosine residues on MAP kinases ERK1 and ERK2. MEK1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. As a key player of the MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.\u003cbr\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.05 mg","offer_id":40383132336209,"sku":"MEK1-B-301","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/MEK1_Protein_Human_Recombinant_Biotinylated.png?v=1736282413"},{"product_id":"wild-type-kras-protein-human-recombinant-biotinylated-without-a-his-tag","title":"Wild Type KRAS Protein, Human, Biotinylated, Without a His-tag","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Arial',sans-serif;\"\u003eRecombinantÊ\u003cb\u003eKRASÊ\u003c\/b\u003eprotein with the N-terminal AviTagª peptide is produced inÊ\u003ci\u003eE. coli\u003c\/i\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\" target=\"_blank\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40505107218513,"sku":"KRAS-B-302","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Wild_Type_KRAS_Protein_Human_Biotinylated_Without_a_His-tag.png?v=1736454446"},{"product_id":"kras-g12v-protein-human-biotinylated-without-a-his-tag","title":"KRAS G12V Protein, Human, Biotinylated, Without a His-tag","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Arial',sans-serif;\"\u003eRecombinantÊ\u003cb\u003eKRASÊG12V \u003c\/b\u003eprotein with the N-terminal AviTagª peptide is produced inÊ\u003ci\u003eE. coli\u003c\/i\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotinylation\" target=\"_blank\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" rel=\"noopener\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":40505107513425,"sku":"KRAS12V-B-302","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12V_Protein_Human_Biotinylated_Without_a_His-tag.png?v=1736454390"},{"product_id":"kras-g12c-y96d-protein-human-recombinant-biotinylated","title":"KRAS G12C, Y96D Protein, Human, Recombinant, Biotinylated","description":"\u003cp\u003e\u003cstrong\u003eKRAS G12C\/Y96D \u003c\/strong\u003edouble mutant displays resistance to multiple KRAS G12C inhibitors currently in clinical development (1). Recombinant \u003cstrong\u003eKRAS G12C\/Y96D \u003c\/strong\u003eprotein with the N-terminal AviTag\u003cmeta charset=\"utf-8\"\u003e\u003cb data-index-in-node=\"18\" data-path-to-node=\"6,0,0\"\u003e™\u003c\/b\u003e peptide and His-tag is produced in \u003cem\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca rel=\"noopener\" title=\"BirA Biotin Ligase | In-vitro Biotinylation | Site-specific | High Activity\" href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotinylation\" target=\"_blank\"\u003eBirA enzyme\u003c\/a\u003e.  \u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":41113491243089,"sku":"KRAS12C-96D-B-301","price":800.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg","offer_id":59460790681681,"sku":"KRAS12C-96D-B-302","price":6000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12C_Y96D_Protein_Human_Recombinant_Biotinylated.png?v=1736454272"},{"product_id":"neurofibromin-nf1-human-recombinant","title":"Neurofibromin (NF1), Human, Recombinant | Amid Biosciences","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eNeurofibromin, the protein encoded by theÊ\u003ci\u003eNF\u003c\/i\u003e1 tumor-suppressor gene, is a GTPase activating protein (GAP) of Ras (Ras-GAP). It downregulates the Ras signaling pathway by promoting the hydrolysis of the active form of Ras (GTP-bound Ras) to an inactive form of Ras (GDP-bound Ras) by increasing the intrinsic GTPase activity of Ras (1).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003e \u003c\/strong\u003eThis product is for research use only. It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.05 mg","offer_id":41917780131921,"sku":"NF1-301","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Neurofibromin_NF1_Human_Recombinant_Amid_Biosciences.png?v=1777752601"},{"product_id":"pi3k-rbd-protein-human-biotinylated","title":"PI3Kα-RBD Protein, Human, Biotinylated","description":"\u003cp style=\"margin-bottom: 0in; line-height: normal;\" class=\"MsoNormal\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Arial',sans-serif;\"\u003ePhosphatidylinositol-3-kinases (PI3Ks) are a family of lipid kinases that mediate a host of cellular functions, including cellular growth, differentiation, motility and survival.ÊThere are several classes and isoforms of PI3K, but only three Class I PI3Ks, PI3K_, PI3K_, and PI3K_, have been shown to be activated by RAS subfamily proteins. Several studies suggest that targeting the RAS\/PI3K_ binding interface may be a clinically effective approach for RAS-driven cancers (1, 2).\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.05 mg","offer_id":42121658237009,"sku":"PIK3A-B-301","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/PI3K_-RBD_Protein_Human_Biotinylated_Amid_Biosciences.png?v=1777752483"},{"product_id":"mek2-protein-human-recombinant-biotinylated","title":"MEK2 Protein, Human, Recombinant, Biotinylated","description":"\u003cp style=\"margin: 0in; background: white;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; color: #333333;\"\u003eMEK2 is a dual-specificity tyrosine\/threonine protein kinase found in the Ras\/Raf\/MEK\/ERK mitogen-activated protein kinase (MAPK) signaling pathway. \u003c\/span\u003e\u003cspan style=\"color: rgb(51, 51, 51); font-family: Arial, sans-serif; font-size: 0.875rem;\"\u003eMEK2 is closely related to MEK1 protein.\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.05 mg","offer_id":42145441546321,"sku":"MEK2-B-301","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/MEK2_Protein_Human_Recombinant_Biotinylated.png?v=1736280556"},{"product_id":"pi3k-rbd-protein-human-gst-tag","title":"PI3Kα-RBD Protein, Human, GST-tag","description":"","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.05 mg","offer_id":58180212654161,"sku":"PI3KA-G-301","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/P13k_-RBD_Protein_Human_GST_Tag.png?v=1757197851"},{"product_id":"ralgds-rbd-human-mbp-tag","title":"RalGDS-RBD, human, MBP-tag","description":"\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0in; line-height: normal;\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Times New Roman',serif;\"\u003eRalGDSÊ(Ral guanine nucleotide dissociation stimulator) is the guanine nucleotide exchange factor (GEF) for the Ras-related protein Ral. Functions as a guanine nucleotide exchange factor (GEF) activating either RalA or RalB GTPases. Interacts and acts as an effector molecule for KRAS, RRAS, HRAS, NRAS and Rap proteins (1, 2).\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":58247828373585,"sku":"RLGDS-301","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/RalGDS-RBD_112625.png?v=1764191624"},{"product_id":"hras-protein-human-biotinylated","title":"HRAS Protein, Human, Biotinylated","description":"\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0in; line-height: normal;\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Times New Roman',serif;\"\u003eRecombinantÊ\u003cb\u003eHRASÊ\u003c\/b\u003eprotein with the N-terminal AviTagª peptide and His-tag is produced inÊ\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":58248319467601,"sku":"HRAS-B-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/HRAS-112625_61d11ffb-08ac-487a-9515-f5ac8f52db5a.png?v=1764191275"},{"product_id":"nras-protein-human-biotinylated","title":"NRAS Protein, Human, Biotinylated","description":"\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0in; line-height: normal;\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Times New Roman',serif;\"\u003eRecombinantÊ\u003cb\u003eNRASÊ\u003c\/b\u003eprotein with the N-terminal AviTagª peptide and His-tag is produced inÊ\u003ci\u003eE. coli\u003c\/i\u003eÊcells and site-specifically biotinylated at AviTag withÊ\u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"0.1 mg","offer_id":58248325529681,"sku":"NRAS-B-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/NRAS-112625.png?v=1764191349"},{"product_id":"nras-g12d-protein-human-biotinylated","title":"NRAS G12D Protein, Recombinant, Human, Biotinylated | Amid Biosciences","description":"\u003cp\u003eRecombinant \u003cstrong\u003eNRAS G12D \u003c\/strong\u003eprotein with the N-terminal AviTag™ peptide and His-tag is produced in \u003cem\u003eE. coli\u003c\/em\u003e cells and site-specifically biotinylated at AviTag with \u003ca href=\"https:\/\/amidbiosciences.com\/products\/bira-biotin-ligase-in-vitro-biotynilation\"\u003eBirA enzyme\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCatalog # NRAS12D-B-301-1\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eNRAS mutations have a very similar effect on cancer cells as KRAS mutations. However, they are much less common. A wild-type NRAS (or normal) protein acts as an “on-and-off switch” involved in controlling cell growth, division, and survival. Mutated NRAS protein acts as an “on switch” resulting in uncontrollable cell growth and division, which can lead to cancer.\u003c\/p\u003e\n\u003cp\u003eActivating mutations in the NRAS gene occur in approximately 2-3% of colorectal carcinomas, 20% of melanomas, and a variety of other human cancers. NRAS mutations are predominantly single nucleotide substitutions, occurring most frequently within exons 2 and 3 at codons 12, 13, and 61. These NRAS mutations are associated with poor clinical response to epidermal growth factor receptor (EGFR) targeted therapies in patients with colorectal cancer. \u003c\/p\u003e\n\u003cp\u003eThe most common NRAS mutations are G12D, G12V, G12C, G12S, G12A, G12R, Q61K, and Q61R. There are currently no approved treatments directly targeting NRAS mutations.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e This product is for research use only. It is not intended for diagnostic or therapeutic use in humans or animals.\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":59412308033617,"sku":"NRAS12D-B-301-1","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/NRAS_G12D_Protein_Recombinant_Human_Biotinylated.png?v=1779476794"},{"product_id":"cyclophilin-a-cypa-kras-g12d-protein-kit","title":"Cyclophilin A (CypA) - KRAS G12D Protein Kit | Molecular Glue Discovery | Amid Biosciences","description":"\u003cp style=\"margin-bottom: 0in; line-height: normal;\" class=\"MsoNormal\"\u003eAmid Biosciences offers \u003cb\u003eCyclophilin A (CypA) - KRAS G12D Protein Kit \u003c\/b\u003eto support discovery of novel inhibitors for targeting \"undruggable\" KRAS mutations (e.g., G12D, G12V) byÊforming a binary complex with chaperone protein cyclophilin A.\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"Kit","offer_id":59414964633681,"sku":"KR12DCP-301","price":1115.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/CypA-KRAS_G12D.jpg?v=1779476561"},{"product_id":"cyclophilin-a-cypa-kras-g12v-protein-kit","title":"Cyclophilin A (CypA) - KRAS G12V Protein Kit | Molecular Glue Discovery | Amid Biosciences","description":"\u003cp\u003eAmid Biosciences offers a specialized \u003cstrong\u003eCyclophilin A (CypA) - KRAS G12V Protein Kit\u003c\/strong\u003e designed to accelerate the discovery and validation of novel small-molecule inhibitors targeting oncogenic RAS mutations. This kit provides a complete set of premium reagents to study the formation of target-chaperone binary complexes and downstream drug-mediated tri-complexes, bypassing the classic \"undruggable\" challenges of smooth RAS surface topography.\u003c\/p\u003e\n\u003ch3\u003eThe Tri-Complex \u0026amp; Molecular Glue Mechanism\u003c\/h3\u003e\n\u003cp\u003eModern oncology frameworks utilize \u003cstrong\u003emolecular glues\u003c\/strong\u003e to force structural interactions between active, GTP-bound mutant RAS proteins and abundant cellular chaperones like Cyclophilin A (CypA). This highly selective binary complex creates a novel, composite binding surface that sterically blocks downstream effector interactions (such as RAF binding), effectively shutting down hyperactivated MAPK signaling pathways in mutant RAS cancers.\u003c\/p\u003e\n\u003ch3\u003eKit Components \u0026amp; Inclusions\u003c\/h3\u003e\n\u003cp\u003eEach kit contains components sufficient for robust in vitro biochemical screening and biophysical characterization:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecombinant Biotinylated Human KRAS G12V (1-185):\u003c\/strong\u003e 0.05 mg | Featuring a specific G12V oncogenic mutation on the switch-sense domain of the isoform KRAS4B.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecombinant Biotinylated Human Wild-Type KRAS (1-185):\u003c\/strong\u003e 0.05 mg | Included for critical counter-screening and selectivity profiling.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecombinant Human Cyclophilin A (CypA) (1-165):\u003c\/strong\u003e 0.1 mg | Tagged with an N-terminal 6XHis tag for versatile assay orientation and capture.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eValidated Assay Applications\u003c\/h3\u003e\n\u003cp\u003eThis multi-protein system is meticulously formulated and optimized for high-throughput screening (HTS) and binding kinetics, including:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSurface Plasmon Resonance (SPR)\u003c\/strong\u003e \u0026amp; Bio-Layer Interferometry (BLI)\u003c\/li\u003e\n\u003cli\u003eAlphaLISA \/ HTRF proximity configurations\u003c\/li\u003e\n\u003cli\u003eTargeted protein degradation (TPD) profiling and complex stability assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProduct Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression Host:\u003c\/strong\u003e All components are expressed and purified from optimized \u003cem\u003eEscherichia coli\u003c\/em\u003e (\u003cem\u003eE. coli\u003c\/em\u003e) systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;90% by Coomassie blue staining on SDS-PAGE.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage \u0026amp; Handling:\u003c\/strong\u003e Shipped on dry ice. Strict storage is required at \u003cstrong\u003e-80°C\u003c\/strong\u003e. To maintain enzymatic and binding integrity, minimize freeze\/thaw cycles and snap-freeze remaining protein in liquid nitrogen after initial aliquotting.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003csmall\u003e\u003cem\u003eCatalog Number: KR12VCP-301 | \u003cstrong\u003eThis product is intended for laboratory research use only (RUO).\u003c\/strong\u003e\u003c\/em\u003e\u003c\/small\u003e\u003c\/p\u003e\n\u003ch3\u003eScientific References\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\u003csmall\u003eSchulze CJ et al. \u003cem\u003eChemical remodeling of a cellular chaperone to target the active state of mutant KRAS.\u003c\/em\u003e Science. 2023;381(6659):794-799.\u003c\/small\u003e\u003c\/li\u003e\n\u003cli\u003e\u003csmall\u003eDeng J, Shen S, Huang L et al. \u003cem\u003eSmall-molecule degraders for oncogenic KRAS G12C and pan-KRAS mutations.\u003c\/em\u003e Nat Commun (2026).\u003c\/small\u003e\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Amid Biosciences","offers":[{"title":"Kit","offer_id":59415758307409,"sku":"KRAS12VCYP-301","price":1115.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/CypA-KRASG12V_Kit_Cancer_Drug_Discovery.jpg?v=1779476475"},{"product_id":"recombinant-human-kras-g12r-protein-c-terminal-avitag-c-terminal-6xhis","title":"KRAS G12R Protein Human, Recombinant, Biotinylated at the C-terminus","description":"\u003cp class=\"MsoNormal\"\u003eThe recombinant KRAS G12R (1-166) protein biotinylated at the C-terminus is designed for high-confidence binding and interaction studies. This construct supports site-specific biotinylation for surface-based assays.\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":59444233240657,"sku":"KRAS12R-B-C-301","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/KRAS_G12_R_Protein_Human_Recombinant_Biotinylated_at_the_C-terminus_Amid_Biosciences.png?v=1779476317"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/collections\/nci-vol-11166-72.jpg?v=1741624118","url":"https:\/\/amidbiosciences.com\/collections\/recombinant-ras-kras-proteins.oembed?page=2","provider":"Amid Biosciences","version":"1.0","type":"link"}