{"title":"Biotinylation Tools \u0026 Recombinant Streptavidins","description":"\u003cdiv class=\"collection-description custom-full-width\"\u003e\n  \n  \u003cp class=\"collection-hero__description\"\u003e\n    Amid Biosciences offers high-quality recombinant \u003cem\u003eBirA Biotin Ligases\u003c\/em\u003e, \u003cem\u003eStreptavidins\u003c\/em\u003e, and vibrant \u003cem\u003eStreptavidin fluorescent conjugates\u003c\/em\u003e engineered for high purity and optimal activity. Our specialized selection supports robust \u003cem\u003ein vitro\u003c\/em\u003e and \u003cem\u003ein vivo\u003c\/em\u003e biotinylation workflows.\n  \u003c\/p\u003e\n\n  \u003ch3 class=\"collection-description__heading\" style=\"margin-top: 20px; margin-bottom: 10px;\"\u003e\n    Key Applications \u0026amp; Features:\n  \u003c\/h3\u003e\n\n  \u003cul class=\"collection-description__list\" style=\"list-style-type: disc; padding-left: 20px;\"\u003e\n    \u003cli style=\"margin-bottom: 8px;\"\u003e\n      \u003cstrong\u003eIn Vivo Biotinylation:\u003c\/strong\u003e Utilize our optimized \u003cem\u003eBL21(λDE3)pBirA\u003c\/em\u003e chemically competent \u003cem\u003eE. coli\u003c\/em\u003e cells for seamless site-specific protein labeling.\n    \u003c\/li\u003e\n    \u003cli style=\"margin-bottom: 8px;\"\u003e\n      \u003cstrong\u003eHigh Affinity Purification:\u003c\/strong\u003e Explore high-capacity Streptavidin Agarose Beads and Magnetic Nanoparticles designed for clean, reliable pull-downs.\n    \u003c\/li\u003e\n    \u003cli style=\"margin-bottom: 8px;\"\u003e\n      \u003cstrong\u003eFlexible Tagging Options:\u003c\/strong\u003e Choose from an array of BirA ligases featuring MBP, GST, or His-tags to match your specific purification requirements.\n    \u003c\/li\u003e\n  \u003c\/ul\u003e\n\n\u003c\/div\u003e","products":[{"product_id":"recombinant-streptavidin-his-tag","title":"Streptavidin, Recombinant, His-Tag | High Purity \u0026 Activity","description":"\u003cp data-path-to-node=\"6,0\"\u003e\u003cb data-path-to-node=\"6,0\" data-index-in-node=\"0\"\u003eRecombinant Streptavidin\u003c\/b\u003e is a homo-tetrameric protein variant (core streptavidin, amino acids 13-139) produced in \u003ci data-path-to-node=\"6,0\" data-index-in-node=\"114\"\u003eE. coli\u003c\/i\u003e and purified using proprietary chromatographic techniques. Consisting of a single, non-glycosylated polypeptide chain of 136 amino acids, it includes an \u003cb data-path-to-node=\"6,0\" data-index-in-node=\"275\"\u003eN-terminal His-Tag\u003c\/b\u003e and features a molecular mass of 14.4 kDa (approximately 57.7 kDa per tetramer).\u003c\/p\u003e\n\u003cp data-path-to-node=\"6,1\"\u003eStreptavidin is widely used in molecular biology due to its extraordinarily high affinity for biotin (\u003cspan class=\"math-inline\" data-math=\"\\text{K}_\\text{d} \\approx 10^{-14} \\text{ mol\/L}\" data-index-in-node=\"102\"\u003e$\\text{K}_\\text{d} \\approx 10^{-14} \\text{ mol\/L}$\u003c\/span\u003e). Because it lacks carbohydrate modifications and maintains a near-neutral pI, it yields significantly lower non-specific binding compared to native avidin.\u003c\/p\u003e\n\u003ch3 data-path-to-node=\"6,2\"\u003eKey Features \u0026amp; Applications:\u003c\/h3\u003e\n\u003cul data-path-to-node=\"6,3\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,3,0,0\"\u003e\u003cb data-path-to-node=\"6,3,0,0\" data-index-in-node=\"0\"\u003eHigh Affinity \u0026amp; Stability:\u003c\/b\u003e The streptavidin-biotin complex remains exceptionally stable across broad pH and temperature ranges.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,3,1,0\"\u003e\u003cb data-path-to-node=\"6,3,1,0\" data-index-in-node=\"0\"\u003eVersatile Workflows:\u003c\/b\u003e Ideal for affinity protein purification, immunoassays, Western blotting, ELISA detection, histochemistry, FISH, and cellular imaging.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,3,2,0\"\u003e\u003cb data-path-to-node=\"6,3,2,0\" data-index-in-node=\"0\"\u003eConvenient Tagging:\u003c\/b\u003e Equipped with an N-terminal His-Tag for seamless detection or secondary immobilization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-path-to-node=\"6,4\"\u003eSpecifications:\u003c\/h3\u003e\n\u003cul data-path-to-node=\"6,5\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,5,0,0\"\u003e\u003cb data-path-to-node=\"6,5,0,0\" data-index-in-node=\"0\"\u003eActivity:\u003c\/b\u003e Greater than 16 U\/mg (1 unit binds 1 µg of D-biotin).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,5,1,0\"\u003e\u003cb data-path-to-node=\"6,5,1,0\" data-index-in-node=\"0\"\u003eStorage Buffer:\u003c\/b\u003e 40 mM Phosphate buffer (pH 7.2), 120 mM NaCl, 0.05% Tween 20, 20% glycerol.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,5,2,0\"\u003e\u003cb data-path-to-node=\"6,5,2,0\" data-index-in-node=\"0\"\u003eFormulation Sequence:\u003c\/b\u003e \u003ccode data-path-to-node=\"6,5,2,0\" data-index-in-node=\"22\"\u003eMHHHHHHKLAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAAS\u003c\/code\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,5,3,0\"\u003e\u003cb data-path-to-node=\"6,5,3,0\" data-index-in-node=\"0\"\u003eCatalog Numbers:\u003c\/b\u003e STR-301-1 (1 mg), STR-301-5 (5 mg), STR-301-25 (25 mg), STR-301-100 (100 mg).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,5,4,0\"\u003e\u003cb data-path-to-node=\"6,5,4,0\" data-index-in-node=\"0\"\u003eIntended Use:\u003c\/b\u003e \u003cstrong\u003eFor research use only (RUO).\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Amid Biosciences","offers":[{"title":"5 mg","offer_id":25626904969,"sku":"STR-301-5","price":550.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":59537185931345,"sku":"STR-301-1","price":200.0,"currency_code":"USD","in_stock":true},{"title":"25 mg","offer_id":59537187405905,"sku":"STR-301-25","price":2200.0,"currency_code":"USD","in_stock":true},{"title":"100 mg","offer_id":59537190682705,"sku":"STR-301-100","price":6820.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Streptavidin_Recombinant_His-Tag_High_Purity_Activity.png?v=1779573023"},{"product_id":"recombinant-bira-biotin-ligase-in-vitro-biotinylation","title":"BirA Biotin Protein Ligase, Recombinant","description":"\u003ch2\u003eRecombinant BirA Biotin Ligase (EC 6.3.4.15)\u003c\/h2\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cspan\u003eBirA (also known as biotin protein ligase or BPL) is widely used for antibody biotinylation, proximity-dependent biotinylation (BioID), and streptavidin\/avidin-based pull-down assays. Because biotinylation is confined to the AviTag sequence (GLNDIFEAQKIEWHE), it avoids the non-specific labeling seen with chemical NHS-ester reagents, preserving protein activity and enabling oriented capture on streptavidin surfaces. \u003cmeta charset=\"utf-8\"\u003eUnlike chemical NHS-ester methods that produce heterogeneous mixtures, BirA achieves site-specific biotinylation exclusively at the AviTag lysine residue.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eKey Features \u0026amp; Specifications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource \u0026amp; Expression:\u003c\/strong\u003e Expressed in \u003cem\u003eE. coli\u003c\/em\u003e cell expression system (amino acids 3–321).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e 36.2 kDa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification Tag:\u003c\/strong\u003e Features an N-terminal His-tag, allowing for easy removal post-reaction using a Ni-NTA column (if the substrate lacks a His-tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;90% as determined by SDS-PAGE (Coomassie staining).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Activity:\u003c\/strong\u003e 5,000 Units\/µg. (1 Unit biotinylates 1 pmol of AviTag peptide substrate in 30 minutes at 30°C).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1.0 mg\/mL (by A280).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eSite-specific \u003cem\u003ein vitro\u003c\/em\u003e biotinylation of target proteins and antibodies.\u003c\/li\u003e\n\u003cli\u003eProximity-dependent biotinylation and cell labeling applications.\u003c\/li\u003e\n\u003cli\u003ePreparation of high-affinity complexes for streptavidin\/avidin pull-downs, immobilization, and functionalization assays.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Amid Biosciences","offers":[{"title":"100 ug","offer_id":12268311478353,"sku":"BBL-301-100","price":240.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/BirA_Biotin_Protein_Ligase_Recombinant.png?v=1779579481"},{"product_id":"high-capacity-streptavidin-agarose-beads","title":"High Capacity Streptavidin Agarose Beads | Amid Biosciences","description":"\u003ch2\u003eHigh Capacity Streptavidin Agarose Resin\u003c\/h2\u003e\n\u003cp\u003eOur \u003cstrong\u003eHigh Capacity Streptavidin Agarose Beads\u003c\/strong\u003e consist of purified streptavidin covalently conjugated to crosslinked 4% agarose beads. Developed using a proprietary coupling procedure, the biotin-binding activity of the immobilized streptavidin is optimally preserved while minimizing ligand leaching and non-specific binding.\u003c\/p\u003e\n\n\u003cp\u003eThis premium chromatography resin is ideal for the rapid, high-yield isolation and separation of biotinylated targets, including recombinant proteins, nucleic acids, antibodies, and small ligands.\u003c\/p\u003e\n\n\u003ch3\u003eKey Features \u0026amp; Specifications:\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHigh Binding Capacity:\u003c\/strong\u003e Binds approximately 30 µg of free biotin per mL of settled resin.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eOptimal Performance:\u003c\/strong\u003e Low non-specific binding and leak-resistant streptavidin immobilization across diverse affinity chromatography conditions.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eVersatile Workflows:\u003c\/strong\u003e Perfectly suited for gravity flow columns, spin columns, and batch purification protocols.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Supplied as a 50% suspension in 0.1 M sodium phosphate (pH 7.2), 0.15 M NaCl, and 0.02% sodium azide (2 mL of slurry equals 1 mL of settled resin).\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eAffinity purification of biotin-tagged proteins and nucleic acid complexes.\u003c\/li\u003e\n  \u003cli\u003eIsolation of Strep-tagged recombinant proteins.\u003c\/li\u003e\n  \u003cli\u003eSolid-phase binding assays and transcription factor pulldowns (e.g., biotinylated DNA probes).\u003c\/li\u003e\n  \u003cli\u003eImmunoprecipitation and antigen purification utilizing biotinylated antibodies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage \u0026amp; Shipping:\u003c\/h3\u003e\n\u003cp\u003eStable for approximately two years when stored at \u003cstrong\u003e2–8°C\u003c\/strong\u003e. \u003cstrong\u003eDO NOT FREEZE\u003c\/strong\u003e, as freezing damages the agarose bead structure. This product requires international shipping on ice packs. Please contact info@amidbiosciences.com for shipping estimates.\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"1 mL","offer_id":28024812873,"sku":"SA-101-1","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/products\/High_Capacity_Streptavidin_Agarose_Beads.jpg?v=1779592137"},{"product_id":"high-purity-streptavidin-recombinant","title":"Streptavidin Recombinant | Amid Biosciences","description":"\u003ch3\u003eRecombinant Streptavidin (Core, High Purity \u0026amp; Activity)\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Streptavidin\u003c\/strong\u003e is a homo-tetrameric protein produced in \u003cem\u003eE. coli\u003c\/em\u003e as an N- and C-terminal shortened variant (\u003cstrong\u003ecore streptavidin\u003c\/strong\u003e, amino acids 13–139). It is purified using proprietary chromatographic techniques to ensure maximum purity and excellent activity.\u003c\/p\u003e\n\u003cp\u003eBecause it lacks carbohydrate modifications and has a near-neutral isoelectric point (pI), our recombinant streptavidin offers significantly lower non-specific binding compared to native avidin, making it ideal for sensitive molecular biology applications.\u003c\/p\u003e\n\n\u003ch3\u003eKey Features \u0026amp; Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Affinity:\u003c\/strong\u003e Binds D-biotin with a dissociation constant (Kd) of ~1.0 x 10^-14 mol\/L, one of the strongest non-covalent interactions in nature.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eExceptional Stability:\u003c\/strong\u003e The streptavidin-biotin complex remains stable across a wide range of temperatures and pH levels.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHigh Activity:\u003c\/strong\u003e Greater than 16 U\/mg (1 unit binds 1 µg of D-biotin).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Supplied as a 5 mg\/mL solution in 0.5X PBS buffer (pH 7.4) and 50% glycerol.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e 13.4 kDa per monomer (single, non-glycosylated polypeptide chain of 128 amino acids); approximately 53.6 kDa per functional tetramer.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cp\u003eOptimized for use in high-performance downstream research applications, including:\u003c\/p\u003e\n\u003cul\u003e\n  \u003cli\u003eEnzyme-Linked Immunosorbent Assays (\u003cem\u003eELISA detection\u003c\/em\u003e)\u003c\/li\u003e\n  \u003cli\u003eFlow Cytometry and Immunohistochemistry\u003c\/li\u003e\n  \u003cli\u003eWestern Blotting and Protein Purification\u003c\/li\u003e\n  \u003cli\u003eFISH (Fluorescence In Situ Hybridization) and Microarrays\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eCatalog # ST-302-5 (5 mg), ST-302-25 (25 mg).\u003c\/em\u003e\u003cbr\u003e\n\u003cstrong\u003eThis product is for research use only (RUO).\u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"5 mg","offer_id":2019419783177,"sku":"ST-302-5","price":550.0,"currency_code":"USD","in_stock":true},{"title":"25 mg","offer_id":59537145856081,"sku":"ST-302-25","price":2200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Streptavidin_Recombinant_High_Purity.png?v=1779758165"},{"product_id":"bl21-de3pbira-e-coli-cells-for-in-vivo-biotinylation","title":"BL21(λDE3)pBirA Competent Cells for in vivo Biotinylation","description":"\u003cblockquote data-path-to-node=\"7\"\u003e\n\u003cp data-path-to-node=\"7,0\"\u003eThe \u003cb data-path-to-node=\"7,0\" data-index-in-node=\"4\"\u003eBL21(λDE3)pBirA strain\u003c\/b\u003e is highly recommended for the expression and \u003ci data-path-to-node=\"7,0\" data-index-in-node=\"72\"\u003ein vivo\u003c\/i\u003e biotinylation of target proteins fused with an \u003cb data-path-to-node=\"7,0\" data-index-in-node=\"127\"\u003eAviTag™ peptide\u003c\/b\u003e or other specific peptide sequences recognized by \u003ci data-path-to-node=\"7,0\" data-index-in-node=\"193\"\u003eE. coli\u003c\/i\u003e BirA biotin ligase.\u003c\/p\u003e\n\u003cp data-path-to-node=\"7,1\"\u003eThis strain features an IPTG-inducible BirA expression plasmid carrying a streptomycin\/spectinomycin resistance gene. It enables high-efficiency protein expression of any gene under the control of a \u003cb data-path-to-node=\"7,1\" data-index-in-node=\"199\"\u003eT7 or E. coli promoter\u003c\/b\u003e equipped with a ribosome binding site. The BirA plasmid is fully compatible for co-expression with popular \u003ci data-path-to-node=\"7,1\" data-index-in-node=\"329\"\u003eE. coli\u003c\/i\u003e and T7 promoter-driven vectors (including pET and pTrc).\u003c\/p\u003e\n\u003cp data-path-to-node=\"7,2\"\u003e\u003cb data-path-to-node=\"7,2\" data-index-in-node=\"0\"\u003eApplications \u0026amp; Usage:\u003c\/b\u003e After transforming the BL21(λDE3)pBirA chemically competent \u003ci data-path-to-node=\"7,2\" data-index-in-node=\"82\"\u003eE. coli\u003c\/i\u003e cells with your target vector (carrying an AviTag™ sequence and an antibiotic resistance gene other than streptomycin\/spectinomycin), grow the cells in the presence of both selection antibiotics. Induce BirA expression using \u003cb data-path-to-node=\"7,2\" data-index-in-node=\"315\"\u003eIPTG\u003c\/b\u003e, and add exogenous biotin to the media at the time of induction at a final concentration of 50 to 200 µM.\u003c\/p\u003e\n\u003cul data-path-to-node=\"7,3\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"7,3,0,0\"\u003e\u003cb data-path-to-node=\"7,3,0,0\" data-index-in-node=\"0\"\u003eCatalog #:\u003c\/b\u003e BLB-201\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"7,3,1,0\"\u003e\u003cb data-path-to-node=\"7,3,1,0\" data-index-in-node=\"0\"\u003eIntended Use:\u003c\/b\u003e\u003cstrong\u003e For laboratory research use only (RUO).\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/blockquote\u003e","brand":"Amid Biosciences| Competent Cells and Protein Expression Vectors","offers":[{"title":"10x0.1 ml (20 reactions)","offer_id":21302001336401,"sku":"BLB-201","price":340.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/BL21BirA.jpg?v=1779571526"},{"product_id":"streptavidin-magnetic-nanoparticles","title":"High Capacity Streptavidin Functionalized Magnetic Nanoparticles","description":"\u003ch2\u003eStreptavidin SilaMag™ Magnetic Nanoparticles\u003c\/h2\u003e\n\u003cp\u003eOur \u003cstrong\u003eHigh Capacity Streptavidin Functionalized Magnetic Nanoparticles\u003c\/strong\u003e (Streptavidin SilaMag NanoBeads) are engineered with an average particle size of 400 nm for the rapid and specific isolation of biotinylated molecules. Manufactured via a modified Stöber process by the hydrolysis of orthosilicates followed by covalent streptavidin coupling, our proprietary functionalization procedure ensures that the biotin-binding activity of the immobilized streptavidin is optimally preserved.\u003c\/p\u003e\n\u003ch3\u003eKey Features:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Binding Capacity:\u003c\/strong\u003e Extensively optimized to bind \u0026gt;1000 pmol of free biotin per mg of particles.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMinimal Background:\u003c\/strong\u003e Low non-specific binding to non-targeted proteins and nucleic acids.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid Kinetics:\u003c\/strong\u003e Fast magnetic separation speeds up workflows and reduces assay times.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePhysical Properties:\u003c\/strong\u003e Uniform 400 nm silica-coated magnetic core structure.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity purification and isolation of biotin-tagged recombinant proteins and ligands.\u003c\/li\u003e\n\u003cli\u003eSolid-phase binding assays and immunoassay development.\u003c\/li\u003e\n\u003cli\u003eNext-Generation Sequencing (NGS) library preparation and nucleic acid separation.\u003c\/li\u003e\n\u003cli\u003eCell separation assays and antigen purification utilizing biotinylated antibodies.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Amid Biosciences","offers":[{"title":"1 mL","offer_id":31941836341329,"sku":"SSMP-101-1","price":90.0,"currency_code":"USD","in_stock":true},{"title":"2 mL","offer_id":59537140056145,"sku":"SSMP-102-2","price":170.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/products\/High_Capacity_Streptavidin_Functionalized_Magnetic_Nanoparticles.jpg?v=1779588880"},{"product_id":"streptavidin-488-fluordye-conjugate","title":"Streptavidin-488 FluorDye Conjugate","description":"\u003cp\u003e\u003cstrong\u003eStreptavidin-488 FluorDye Conjugate\u003c\/strong\u003e is a bright, green-emitting fluorescent conjugate engineered for the highly specific detection of biotin-labeled proteins, antibodies, nucleic acids, and motifs in diverse assay formats. Utilizing a high-performance fluorophore structurally and spectrally equivalent to Alexa Fluor® 488, this conjugate delivers exceptional photostability and intense fluorescence signals.\u003c\/p\u003e\n\u003cp\u003eOur conjugation process features an optimized fluorophore-to-protein labeling ratio, ensuring maximum fluorescence intensity while fully preserving the native binding affinity of streptavidin.\u003c\/p\u003e\n\u003ch3\u003eKey Features \u0026amp; Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpectral Profiles:\u003c\/strong\u003e Absorbance maximum at 494 nm; Emission maximum at 517 nm (Green spectrum).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Supplied as a 1 mg\/mL solution in 0.5X PBS buffer (pH 7.4) and 50% glycerol.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDye Equivalence:\u003c\/strong\u003e 488 FluorDye is an ideal alternative to Alexa Fluor® 488.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e Store at -20°C. Protect from light to prevent photobleaching.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping Notice:\u003c\/strong\u003e Requires shipping on dry ice. Please contact info@amidbiosciences.com for shipping estimates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cp\u003eValidated for a wide range of high-performance downstream imaging and molecular biology platforms:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eFlow Cytometry (FACS)\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eImmunohistochemistry (IHC) \u0026amp; Immunocytochemistry (ICC)\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern Blotting\u003c\/strong\u003e (Fluorescent detection systems)\u003c\/li\u003e\n\u003cli\u003eImmunofluorescence Microscopy and Microplate Assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol Notes \u0026amp; Handling Guidelines\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSuggested Working Dilution Range:\u003c\/strong\u003e 0.5–2 µg\/mL for both Western Blotting and Histo-\/Cyto-Chemistry. Because optimal dilution is a function of antigen density and sample permeability, actual concentrations must be determined empirically by the user.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEndogenous Biotin Blocking:\u003c\/strong\u003e It is necessary to block endogenous biotin prior to staining samples to minimize background noise and avoid false positives.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackground Optimization:\u003c\/strong\u003e If non-specific background binding is high, reduce the working concentration. Alternatively, briefly centrifuge the conjugate vial and use only the clear supernatant for your staining protocol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eCatalog # STFL-302-1 (1 mg), STFL-302-2 (5 mg).\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eThis product is for laboratory research use only (RUO). \u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"1 mg","offer_id":32094244372561,"sku":"STFL-302-1","price":250.0,"currency_code":"USD","in_stock":true},{"title":"5 mg","offer_id":59537148575825,"sku":"STFL-302-2","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Streptavidin-488_FluorDyeConjugate.png?v=1736365877"},{"product_id":"streptavidin-568fluordye-conjugate","title":"Streptavidin-568 FluorDye Conjugate","description":"\u003cp\u003eStreptavidin-568 FluorDye conjugate is an orange-emitting conjugate used for specific detection of a variety of biotin labeled proteins, antibodies, protein motifs, nucleic acids, and other molecules.\u003c\/p\u003e\n\u003cp\u003eStreptavidin-568 FluorDye conjugate was optimized in fluorophore\/protein labeling ratio to ensure high fluorescent signal and uncompromised streptavidin function. 568 FluorDye is equivalent to Alexa Fluor® 568.\u003c\/p\u003e\n\u003cp\u003eSuggested applications: western blots, flow cytometry, imaging and microscopy, microplate assays and more.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCatalog # STFI-304\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThis product is for laboratory research use only.\u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"1 mg","offer_id":32094278778961,"sku":"STFL-303-1","price":250.0,"currency_code":"USD","in_stock":true},{"title":"5 mg","offer_id":59537152147537,"sku":"STFL-303-2","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Streptavidin-568_FluorDyeConjugate.png?v=1736365956"},{"product_id":"streptavidin-647-fluordye-conjugate","title":"Streptavidin-647 FluorDye Conjugate","description":"\u003cp\u003e\u003cstrong\u003eStreptavidin-647 FluorDye Conjugate\u003c\/strong\u003e is a bright, far-red-emitting fluorescent conjugate optimized for the highly specific detection of biotin-labeled proteins, antibodies, nucleic acids, and motifs. Utilizing a premium long-wavelength dye equivalent to Alexa Fluor® 647, this conjugate offers exceptionally low autofluorescence in biological specimens, ensuring a superior signal-to-noise ratio.\u003c\/p\u003e\n\u003cp\u003eOur conjugation process carefully optimizes the fluorophore-to-protein labeling ratio to guarantee intense fluorescent signals without compromising native streptavidin binding affinity.\u003c\/p\u003e\n\u003ch3\u003eKey Features \u0026amp; Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpectral Profiles:\u003c\/strong\u003e Absorbance maximum at 650 nm; Emission maximum at 665 nm (Far-Red spectrum).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Supplied as a 1 mg\/mL solution in 0.5X PBS buffer (pH 7.4) and 50% glycerol.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDye Equivalence:\u003c\/strong\u003e 647 FluorDye is structurally and spectrally equivalent to Alexa Fluor® 647.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLabeling Efficiency:\u003c\/strong\u003e Engineered with an optimized fluorophore-to-protein labeling ratio to ensure an intense fluorescent signal without compromising native streptavidin binding affinity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e Store at -20°C. Protect from light.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping Notice:\u003c\/strong\u003e Ships on dry ice. Contact info@amidbiosciences.com for shipping estimates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cp\u003eIdeal for high-performance downstream imaging and molecular assay platforms, including:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eFlow Cytometry (FACS)\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eImmunohistochemistry (IHC) \u0026amp; Immunocytochemistry (ICC)\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern Blotting\u003c\/strong\u003e (Fluorescent detection)\u003c\/li\u003e\n\u003cli\u003eMicroscopy, Cellular Imaging, and Microplate Assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProtocol Notes \u0026amp; Handling\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSuggested Working Range:\u003c\/strong\u003e 0.5–2 µg\/mL for Western Blotting and Histo-\/Cyto-chemistry. Optimal dilutions must be determined empirically based on target antigen density and permeability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEndogenous Biotin:\u003c\/strong\u003e It is necessary to block endogenous biotin prior to staining samples to minimize background noise.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackground Minimization:\u003c\/strong\u003e If non-specific background is high, decrease the working concentration or briefly centrifuge the conjugate, using only the clear supernatant for staining.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003eCatalog # STFI-304-1 (1 mg), STFL-304-2 (5 mg).\u003c\/em\u003e\u003cbr\u003e\u003cstrong\u003eThis product is for laboratory research use only (RUO). \u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"1 mg","offer_id":32094315446353,"sku":"STFl-304-1","price":250.0,"currency_code":"USD","in_stock":true},{"title":"5 mg","offer_id":59537162895441,"sku":"STFL-304-2","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/Streptavidin-647_FluorDyeConjugate.png?v=1736366033"},{"product_id":"bira-biotin-ligase-mbp-tag-recombinant","title":"BirA Biotin Ligase, MBP-tag, Recombinant","description":"\u003ch3\u003eHigh-Purity Recombinant BirA Biotin Ligase for Site-Specific Labeling\u003c\/h3\u003e\n\u003cp\u003eAmid Biosciences presents high-purity \u003cstrong\u003erecombinant BirA Biotin Ligase\u003c\/strong\u003e (~78 kDa), expressed as a maltose-binding protein (MBP) fusion in \u003cem\u003eE. coli\u003c\/em\u003e cells. This versatile enzyme is engineered with a \u003cstrong\u003edual 6xHis-MBP tag at the N-terminus\u003c\/strong\u003e, enhancing protein solubility and allowing for effortless purification or removal post-biotinylation using Ni-NTA or amylose resin columns.\u003c\/p\u003e\n\u003ch3\u003eKey Applications \u0026amp; Performance\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePrecise Site-Specific Biotinylation:\u003c\/strong\u003e Ideal for highly efficient, targeted biotin labeling of proteins and peptides containing an AviTag™ or identical peptide substrate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile Workflows:\u003c\/strong\u003e Optimized for both rigorous \u003cem\u003ein vitro\u003c\/em\u003e enzymatic labeling and \u003cem\u003ein vivo\u003c\/em\u003e proximity labeling applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Specific Activity:\u003c\/strong\u003e Offers robust performance with \u0026gt;3,000 Units\/µg, ensuring reliable results for your molecular biology and assay development needs.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eCatalog # MBBL-301\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eThis product is intended for research use only (RUO).\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":32399019016273,"sku":"MBBL-301","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/BirA_Biotin_Ligase_MBP-tag_Recombinant.png?v=1779731291"},{"product_id":"bira-biotin-ligase-gst-tag","title":"BirA Biotin Ligase, GST-tag","description":"\u003cp\u003eAmid Biosciences offers high-purity \u003cstrong\u003eBirA Biotin Ligase enzyme\u003c\/strong\u003e, recombinantly expressed with an N-terminal 6XHis and glutathione S-transferase (GST) dual tag in \u003cem\u003eE. coli\u003c\/em\u003e. This innovative dual-tag architecture allows for effortless, flexible removal of the BirA enzyme following your biotinylation reaction using either Ni-NTA or glutathione agarose resin columns, ensuring a pristine end product.\u003c\/p\u003e\n\n\u003cp\u003eOur BirA biotin ligase is optimized for highly precise, site-specific protein biotinylation. It is a critical tool for researchers utilizing proximity labeling, AviTag™ sequence targeting, and advanced molecular assay developments that require highly selective covalent labeling of target proteins.\u003c\/p\u003e\n\n\u003ch3\u003eKey Features \u0026amp; Specifications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eExpression Host:\u003c\/strong\u003e \u003cem\u003eEscherichia coli (E. coli)\u003c\/em\u003e\n\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eExpressed Region:\u003c\/strong\u003e Asp3–Lys321 (UniProtKB - P06709; BIRA_ECOLI)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDual-Tag System:\u003c\/strong\u003e N-terminal 6XHis and GST tags (~64 kDa total molecular weight) for streamlined downstream enzyme depletion.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHigh Activity:\u003c\/strong\u003e \u0026gt;3,000 Units\/µg.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eActivity Definition:\u003c\/strong\u003e 1 Unit is defined as the amount of enzyme that will biotinylate 1 pmol of AviTag peptide substrate in 30 minutes at 30°C in optimal reaction buffer conditions.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFormulation \u0026amp; Buffer:\u003c\/strong\u003e Supplied in 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 3 mM DTT, and 50% Glycerol.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cp\u003eValidated for high-performance downstream research applications across in vitro and in vivo platforms, including:\u003c\/p\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSite-Specific Biotinylation\u003c\/strong\u003e of AviTag™ or Biotin Acceptor Peptide (BAP) tagged proteins.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProximity Labeling\u003c\/strong\u003e and interactome mapping studies.\u003c\/li\u003e\n  \u003cli\u003ePreparation of biotinylated targets for Streptavidin-based pull-downs, ELISAs, and surface plasmon resonance (SPR).\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage \u0026amp; Handling Guidelines\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eStorage Conditions:\u003c\/strong\u003e Recommended storage at -20°C for extended stability.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eShipping Notice:\u003c\/strong\u003e Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipping estimates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eCatalog # BBL-G-301 (0.1 mg).\u003c\/em\u003e\u003cbr\u003e\n\u003cstrong\u003eThis product is for laboratory research use only (RUO).\u003c\/strong\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"0.1 mg","offer_id":41987213492305,"sku":"BBL-G-301","price":400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/BirA_Biotin_Ligase_GST-tag.png?v=1736283881"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/collections\/BL21BirA.jpg?v=1779571646","url":"https:\/\/amidbiosciences.com\/collections\/biotinylation-tools-and-recombinant-streptavidins.oembed","provider":"Amid Biosciences","version":"1.0","type":"link"}